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目的应用PCR-DGGE(denaturing gradient gel electrophoresis,变性梯度凝胶电泳)技术对孤独症家庭成员肠道微生物菌群结构进行研究.材料和方法提取粪便细菌总基因组DNA,PCR扩增细菌16S rDNA基因V3可变区,DGGE方法检测PCR产物.结果建立了孤独症家庭成员肠道菌群组成的DGGE指纹图谱,分析结果显示孤独症儿童肠道菌群结构的相似度为0.42-0.65,家庭内孤独症儿童与父母之间的相似度为0.42-0.68.结论 PCR-DGGE技术是一种快速有效的用于分析研究人体肠道菌群结构的技术.孤独症儿童肠道菌群结构与父母的相似度较高,提示生活环境对肠道菌群的组成有影响.
Abstract：PCR-DGGE(denaturing gradient gel electrophoresis) was used to analyze the diversities of intestinal bacteria in sixteen individuals from seven autism families.DGGE analysis showed that the similarity values of the comparisons of the bacterial DGGE bands patterns of the autistic individuals ranged from 0.42 to 0.65,and the autistic children and their parents in the family ranged from 0.42 to 0.68.PCR-DGGE was proved to be a powerful tool to analyze the intestinal bacterial diversity.There existed a correlation between the intestinal bacteria and the living environment.
[1]Johnson,C.P.and Myers,S.M.Identification and evalua-tion of children with autism spectrum disorders.Pediatrics,2007,120:1183-1215.
[2]Yeargin-Allsopp,M.et al.Prevalence of autism in a USmetropolitan area.J.Am.Med.Assoc.2003,289:49-55.
[3]Gurney,J.G.et al.Parental report of health conditions andhealth care use among children with and without autism:National Survey of Children’s Health.Arch.Pediatr.Ado-lesc.Med.2006,160:825-830.
[4]Joseph Piven,M.D.,Pat Palmer,Ph.D,et al.Broader Au-tism Phenotype:Evidence From a Family History Study ofMultiple-Incidence Autism Families.Am J Psychiatry.1997,154:185-190.
[5]James S.Sutcliffe.Insights into the Pathogenesis of Au-tism.AAAS.2008,321:208-209.
[6]Bolte ER.Autism and Clostridium tetani.Med Hypotheses.1998,51(2):133-44.
[7]Finegold SM.Therapy and epidemiology of autism———clos-tridial spores as key elements.Med Hypotheses.2008,70(3):508-11.
[8]Sydney M.Finegold.Therapy and epidemiology of autism-clostridial spores as key elements.Medical Hypotheses,2008,70:508-511.
[9]Relman DA,Schmidt TM,MacDermott RP,et al.Identifica-tionof the uncultured bacillus of Whipple s disease[J].NEngl JMed,1992,327(5):293-2301.
[10]卢珊,孙晖,熊衍文,周永运,叶长芸.婴幼儿腹泻粪便标本菌群的16S rDNA PCR-DGGE分析.中国人兽共患病学报,2009,25(3).
[11]李可俊,管卫兵,徐晋麟,张延,赵立平.PCR-DGGE对长江河口八种野生鱼类肠道菌群多样性的比较研究.中国微生态学杂,2007,19(3).
[11]陈蓓,黄瑞.粪便标本中细菌DNA提取方法的比较.中国血液流变学杂志,2007,17(2):210-214.
[13]Muyzer,G.E;C.de Waal;A.G.Uitterlinden.Profilingof complex microbial populations by denaturing gradient gelelectrophoresis analysis of polymerase chain reaction-ampli-fied genes coding for 16S rRNA.Appl Environ Microbiol.1993,59:695-700.
[14]McAuliffe L,Lawes J,Bell S,et al.The detection of Myco-plasma(formerly Eperyt hrozoon)wenyonii by 16S rDNAPCR and denaturing gradient gel elect rophoresis[J].VetMicrobiol,2006,117(224):292-296.
[15]Apostolov E,AlSoud WA,Nilsson I,et al.Helicobacter py-loriand other Helicobacter species in gallbladder and liverof patient swith chronic cholecystitis detected by immuno-logical and molecular met hods[J].Scand J Gastroenterol,2005,40(1):96-102.
[16]王保红,李?等.一四代同堂庭肠道菌群群结构和代谢组模式特征的研究.2006.
[17]Les Dethlefsen,Paul B.Eckburg,Elisabeth M.Bik andDavid A.Relman.Assembly of the human intestinal mi-crobiota.TRENDS in Ecology and Evolution.2006,21(9):517-523.
基本信息:
DOI：
中图分类号:R749.94
引用信息:
[1]张仁敏,周东蕊,宋媛等.PCR-DGGE技术分析孤独症家庭成员肠道菌群结构[J].南京晓庄学院学报,2010,26(06):66-69.
基金信息:
国家高新技术(863)项目(编号:2006AA020702);; 国家自然基金项目(编号:30600152)